Journal: In Vivo

Article Title: Exercise Stimulates PINK-1, PARKIN, MFN-1 , and ATG-3 Genes Expression Despite High-fat Diet: Tissue-specific Responses

doi: 10.21873/invivo.14298

Figure Lengend Snippet: The body and tissue weight of mice in the study groups. After 24 weeks of feeding and exercise, the body and tissue weight of the mice in the highfat diet (HFD) group are dramatically higher than that of the control and exercise and high-fat diet group (E-HFD) group. A) the total body weight (g) of mice in each experimental group. B) Individual organ and tissue weights (g), including the heart, adipose tissue, and liver for each mouse. C) Compares organ weights (g) across the experimental groups. All values represent individual animals, and units are reported in grams (g). C: Control group.

Article Snippet: Both E-HFD and HFD groups were provided a diet containing 60% fat kcal, the control group was given a diet consisting of 10% kcal of fat (Catalog No: #58Y2, 58Y1; Purina and TestDiet, St. Louis, MO, USA).

Techniques: Control

Journal: In Vivo

Article Title: Exercise Stimulates PINK-1, PARKIN, MFN-1 , and ATG-3 Genes Expression Despite High-fat Diet: Tissue-specific Responses

doi: 10.21873/invivo.14298

Figure Lengend Snippet: The log 2 FC of ATG3, PINK1, PARKIN and MFN1 expression in the liver, adipose and heart tissue of mice in the study groups. Exercise and highfatdiet group (E-HFD) and high-fat diet group (HFD) groups are compared to the control group, while the comparison between the exercise and HFDgroups is conducted relative to the HFD group. Data are shown as log 2 fold change (log 2 FC). C: Control group.

Article Snippet: Both E-HFD and HFD groups were provided a diet containing 60% fat kcal, the control group was given a diet consisting of 10% kcal of fat (Catalog No: #58Y2, 58Y1; Purina and TestDiet, St. Louis, MO, USA).

Techniques: Expressing, Control, Comparison

Journal: In Vivo

Article Title: Exercise Stimulates PINK-1, PARKIN, MFN-1 , and ATG-3 Genes Expression Despite High-fat Diet: Tissue-specific Responses

doi: 10.21873/invivo.14298

Figure Lengend Snippet: Proinflammatory cytokine serum levels and correlation analyses of their relationship with mitophagy-related genes. A) The serum levels of TNF-α, IL-6 and IL-1B in the high-fat diet (HFD) and control groups. B) TNF-α, IL-6 and IL-1B correlation with ATG3, PINK1, PARKIN and MFN1 in the heart tissue of the HFD group. C) TNF-α, IL-6 and IL-1B correlation with ATG3, PINK1, PARKIN and MFN1 in the liver tissue of the HFD group. D) TNF-α, IL-6 and IL-1B correlation with ATG3, PINK1, PARKIN and MFN1 in the adipose tissue of the HFD group. C: Control group.

Article Snippet: Both E-HFD and HFD groups were provided a diet containing 60% fat kcal, the control group was given a diet consisting of 10% kcal of fat (Catalog No: #58Y2, 58Y1; Purina and TestDiet, St. Louis, MO, USA).

Techniques: Control

Journal: PLoS ONE

Article Title: Effect of Dietary Advanced Glycation End Products on Mouse Liver

doi: 10.1371/journal.pone.0035143

Figure Lengend Snippet: AGE content in mouse diets.

Article Snippet: TestDiet 58G7 , 60649±1147 , 1107±39.

Techniques:

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 2 Nogo-B deficiency alleviates weight gain and impaired glucose tolerance in NAFLD model mice. (A) Photos of the mice and their livers at the end of the 12-week experiment; (B) body weight (weekly); (C) body weight (last measurement); (D) liver weight (last measurement); (E) glucose tolerance test (GTT); (F) AUC of the GTT; (G) insulin tolerance test (ITT); (H) AUC of the ITT. n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. NCD, normal chow diet; HFD, high-fat diet; AUC, area under the curve; BMDMs, bone marrow-derived macrophages; WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Derivative Assay

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 3 Nogo-B deficiency mitigates liver lipid accumulation and liver injury in NAFLD model mice. (A) Serum triacylglycerol (TG); (B) serum total cholester ol (TC); (C) serum low-density lipoprotein cholesterol (LDL-C); (D) serum high-density lipoprotein cholesterol (HDL-C); (E) serum alanine aminotransferase (ALT); (F) serum aspartate aminotransferase (AST); (G) serum alkaline phosphatase (ALP); (H) serum albumin (ALB); (I) hematoxylin and eosin (H&E) stain ing of liver sections (scale bar: 200 μm); (J) oil red O staining of liver sections (scale bar: 200 μm). n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Staining

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 4 Nogo-B deficiency attenuates inflammation in the livers of NAFLD model mice. (A) The concentration of interleukin-6 (IL-6); (B) the concentration of tumor necrosis factor-α (TNF-α); (C) the mRNA expression of tumor necrosis factor-α (TNF-α); (D) the mRNA expression of monocyte chemoattractant protein-1 (MCP1); (E) the mRNA expression of CD80; (F) the mRNA expression of CD86; (G) the mRNA expression of arginase-1 (Arg1); (H) the mRNA ex pression of arginase-1 (Arg1); (I) the mRNA expression of macrophage galactose N-acetyl-galactosamine specific lectin-2 (Mgl2); (J) the mRNA expression of resistin-like alpha (Retlna). n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Concentration Assay, Expressing

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 6 Nogo-B deficiency regulates serum metabolites in NAFLD model mice. (A) Principal component analysis (PCA) score plot; (B) partial least squares discriminant analysis (PLS-DA) score plot; (C) orthogonal partial least squares discriminant analysis (OPLS-DA) score plot; (D) OPLS-DA permutation test; (E) volcano plot, in which the red spots represent the significantly upregulated metabolites (log2FC > 0), the blue spots represent the significantly down regulated metabolites (log2FC < 0), and the gray spots represent the nonsignificant metabolites (P > 0.05); (F) clustered heatmap and VIP value bar graphs of differentially abundant metabolites (top 30 VIP values) between the WT-HFD and Nogo-B−/−-HDF groups; (G) KEGG pathways (top 20 enrichment ra tios) of differentially abundant metabolites. n = 5. VIP, variable importance in the projection; WT, wild-type mice; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: